ABSTRACT
Introduction@#The main goal of the modern national drug manufacturer is to develop highly biological active, low-side effects and no toxicity pharmaceutical products that can replace synthetic drugs with natural animal, plant and mineral raw materials. We conducted this study in aim to develop the standard of pharmacopoeia of root of <i>Caryopteris mongolica</i> which an antibacterial activity plant that grows in Mongolia. The study included experiments to determine the appearance of the plant, to determine its anatomical structure, to determine the quantitative analysis of pharmacognosy and to determine the microbiological analysis.@*Method@#We defined the appearance of plant by sensory method, anatomical structure of plant by light microscopy method. And we experimented the quantitative analysis of pharmacognosy and microbiological analysis of plant according to the methodology specified in the general requirements for medicinal plant raw material of the National Pharmacopoeia of Mongolia.@*Conclusion@#As a result of the study, the appearance of plant was brown, woody thick, has many branched roots, odorless and bitter. The quantitative analysis of pharmacognosy of plant met the general requirements for medicinal plant raw material. For microbiological analysis, plant met the all requirements.
ABSTRACT
Introduction: Alzheimer’s disease(AD) is the most common neurodegenerative disease and characterized by memory impairment, language deterioration, cognitive dysfunction, behavioral disturbances and deficits in activities in daily living [1,2]. Acetylcholinesterase(AchE) inhibitors have been used effective treatment for AD disease targets[3]. Therefore, it is needed to isolate bioactive compounds from plants.Materials and methods: Trisma buffer pH 8.0, DTNB(5.5-dithiobis-2-nitribenzoic acid), acethylcholinesterase enzyme, acethylcholin iodide- Ach and methanol reagents were used. Plants were collected from Mongolian khangai, steppe, gobi regions. The dried plant material were extracted with methanol then evaporiated at 400C in vacu. AChE inhibitory activity was determined using the Ellman’s colorimetric method.Calculation:A10 min-A00min= Δ A Inhibition % =100x (A10 control- Δ A sample) A10control A=absorptionResults and discussion: From the 65 samples studied, which prepared from different parts of 44 plants species, Salsola passerine, Rumex pseudonatronatus, Solidago dahurica, Chelidonium majus, Anthriscus sylvestris, Linaria buriatica and Achnatherum splendens showed more effect, their inhibition percentages were 32%, 30%, 30,7%, 35,7%, 61,5%, 29,8%, 28.8% and26.6%, respectively. Out of the plants, eight extracts were not identified AChE inhibitory activity and 30 extracts showed 10-20% weak inhibition ratios. This work represents some Mongolian plants have AChE inhibitory activity.Referens:1. Marston A., Kissling J., Hostettmann K., A rapid TLC bioautographic method for the detection of acetylcholinesterase and butyrylcholinesterase inhibitors in plants.Phytochem.Anal. 13.51- 54(2002)2. Eduardo luis Konrath., Bruna Medeiros Neves et all., Investigations of the in vitro and in vivo acetylcholinesterase and antioxidant activities of tradionally used Lycopodium species from South America on alkaloid extracts. Journal of Ethnoparmacology 139(2012)58-673. Shulz V., Ginkgo extract or cholinesterase inhibitors in dementia., Phytomedicine 10(2003)74-79
ABSTRACT
Acethylcholinesterase (AChE) inhibitors have been used in the treatment of Alzheimer’s disease (AD). Currently, the only effective treatment for AD disease targets the cholinergic system using anti-cholinesterase compounds. AD is progressive, degenerative disease characterized by memory loss, language deterioration, poor judgment, impaired visuospatial skills etc. There are a few synthetic medicines, e.g. tacrine, donepezil and the natural product-based rivastigmine and galantamine to treat cognitive dysfunction and memory loss associated with AD. These approved drugs are limited in use due to their adverse side effects such as gastrointestinal disturbance and bioavailability problems. There still is great interest in fi nding better AchE inhibitors. We have screened in this study the anti-acethylcholinesterase effects of 87 samples, which prepared from different parts of 43 plant species. Methanolic extracts of nine plants, namely Patrinia rupestris, Numpaea candida, Lespedeza hedysaroides, Pachypfeurum alpinum, Leontopodium Leontopodioides, Nonea pulla, Leontopodium compestre, Gypsophilea patrinii and Abies sibirica showed potent effect, their inhibition ratios were 39.65%, 42.22%, 38.06%, 40.55%, 38.82%, 35.1%, 35.85%, 35.3 % and 53.01%, respectively. Our research shows that some Mongolian medicinal plants have anti-acetylcholinesterase activity, furthermore investigations needed to isolate pure bio-active compounds from these active plants and determine their chemical structures.